Melonet DB
for functional genomics research of muskmelon
gene ID search :
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[Last update: 28th, Oct, 2023]
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Harukei-3 ver1.41
TASUKE+
320 lines from public DB
Expression atlas
Co-expression
GO analysis
Sequence analysis
Blast+
Short DNA mapper
Clustalw (fasta input)
Primer finder
Tm calculation
Download
Primer search tool
for target sequence
query (input):
! Enter target DNA sequence like below. For trial, just click "Search" button. ATGACCACTCTCTCTCAAACGTATCGAGACCACCCTCTTATTCACCATCACCATATCGTTCCTTTAGACTTTGATTCATTACGAACGATTCCTGATTCTCACGATTGGCTCCATTCCTCCCCTGAAACCCCTTCTTCTTCTTCTTCTTCTTCTTGTTATCATACTAATGTAGATGTTTCAATCCCTTTGATTGATCTGACTGACCATGATGCAATCGCGCTCATCGGCAATGCATGCGAGACGTGGGGAGTGTTCCAATTGATCAACCACGACGTGCCAATGAGCCTAATAGAGAAAGCTGAAGGCGAAACGAGACGACTGTTCGATCTTCCGATGACACGAAAGCTGAAGGCACTTCGGACTCCAAGAGACGTCACTGGATATGGATTGCCTCGAATAACGCCATTTTTCTCTAAGTATATGTGGCATGAAGGGTTTACTATTATGGGGCCTCCCATTGATCATGCCTCACAGCTTTGGCCTTCTAACTACCAACCGTTTTGCGATGTGATGGAAGAGTATCAAAGGAAAATGAAGGCCTTAGCGGAGCAAATCACAAATTTAATCTTCAATTACCTTAAAATCTCCGACTGCGCAAACTGGCTCCATTCAACCGGAGCAGCCGCCGCCTGCAGCACCGCCCTTCAACTTAACTGCTACCCGCGCTGTCCGGACCCGACTCGTGTCATGGGCTTGGCCCCACACACCGACACGTTTCTATTGACCATCCTCCACCAGACCCGCACCTGCGGCCTCCAAATTTTCAGGGATGGGTCCGGCTGGGTACCGGTGGCGCCGCTACCCGGTGCGCTGGTCCTCAACGTCGGGGACTTGTTCCACATCCTTTCCAATGGGCGGTTCCCGAATGTTTTACACCGGGTCGTTGTTGACCCGACTCAACGCCGGCTGTCTATGGCTTATTTCTACGGGCCGCCACCGGATTTCTGTGTATCGCCGTTGAATGATCCACCGGAAACTCCCTGTTACCGGCCTGTGGTGGTGAAGGATTATGTTCGGTTGAAGGCCAAGAATCTCGAAAATGCCCTTTCAATGATCCGACTATGA
thresholds:
Tm value :
to
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primer length (bp) :
to
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GC% :
to
seeking primers within:
bp from 5' end
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bp from 3' end (*based on the 5'-position of Fw and Rv primers)
primer uniqueness analysis:
Melon, Cucumis melo [Harukei-3 genome reference, ver1.41]
Melon, Cucumis melo [DHL92 genome reference, CM3.6.1]
Melon, Cucumis melo [DHL92 genome reference, CM3.5.1]
Tomato, Solanum lycopersicum [Heinz1706 genome reference, SL4.0]
Tomato, Solanum lycopersicum [Heinz1706 genome reference, SL3.0]
Arabidopsis thaliana [Col-0, TAIR10 genome reference]
--not using any genome reference to check uniqueness of primers--
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