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for functional genomics study of muskmelon
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Harukei-3 ver1.41
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public data
Expression atlas
Co-expression
GO analysis
e-TILLING
Sequence analysis
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Clustalw (fasta input)
Primer finder
Primer finder
Tm calculation
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Univ. of Tsukuba
[Last update: 8th, Dec, 2022]
Primer search tool
    
for target sequence
query (input):
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thresholds:
Tm value :
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   primer length (bp) :
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   GC% :
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seeking primers within:
bp from 5' end  
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bp from 3' end  (*based on the 5'-position of Fw and Rv primers)
primer uniqueness analysis:
Melon, Cucumis melo [Harukei-3 genome reference, ver1.41]
Melon, Cucumis melo [DHL92 genome reference, CM3.6.1]
Melon, Cucumis melo [DHL92 genome reference, CM3.5.1]
Tomato, Solanum lycopersicum [Heinz1706 genome reference, SL4.0]
Tomato, Solanum lycopersicum [Heinz1706 genome reference, SL3.0]
Arabidopsis thaliana [Col-0, TAIR10 genome reference]
--not using any genome reference to check uniqueness of primers--
Search
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